HJS864147

货号 规格 价格(¥)
HJS864147S 50ug
HJS864147M 100ug
HJS864147L 1000ug

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Note:  Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.

注意:抗体应用为IHC的,抗体只适合于石蜡切片的组化染色或者荧光染色。

抗体应用为IF/ICC的,抗体适合于冰冻切片的组化染色或者荧光染色,以及细胞水平的化学染色和荧光染色。

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   蛋白质免疫共沉淀

   蛋白质印迹

   免疫荧光

   免疫组化

   膜再生

   植物BPP法变性蛋白提取

   组织制备成单细胞悬液

经典一抗-ZEBOV NP/Nucleoprotein Antibody(KZ297)

Datasheet

Background

Description

Oligomerizes into helical capsid to encapsidate the viral genome, protecting it from nucleases and the cellular innate immune response . VP35 binds to and stabilizes monomeric NP, keeping it soluble . Upon virus replication, NP is recruited to bind cooperatively viral genomic RNA and VP35 is released . The encapsidated genomic RNA is termed the nucleocapsid and serves as template for transcription and replication. The nucleocapsid is helical with a pitch of 10.81 NP per turn and a diameter of about 22nm . Each NP binds to six nucleotides of viral genomic RNA, three being exposed to the solvant and three hidden into the nucleocapsid . Also recruits host PPP2R5C phosphatase to dephosphorylate VP30 and thereby promote viral transcription . Upon virion assembly and budding, NP binds to VP24 and possibly host STAU1 . .

Uniprot

p18272

Alternative Names

Nucleoprotein (Ebola NP) (eNP) (Nucleocapsid protein) (Protein N),NP,NP, Nucleoprotein, Nucleocapsid protein

Specificity

Application Image

Reactivity

Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus)

Application

IF,ICC,ELISA

Recommended Dilution

IF 1:100-1:500
ICC 1:100-1:400
ELISA 1:5000-1:20000

Mol weight

84kD

Conjugation

Source

Human

Immunogen

Clonality

Monoclonal

Clone No.

KZ297

Isotype

IgG

Purification

Protein A/G purified from cell culture supernatant.

Purity

>95% as determined by SDS-PAGE.

Buffer

0.01M PBS, pH 7.4.

Concentration

1 mg/ml

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.

Note

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      微信客服

邮箱:market@ab-mart.com

应聘职位:hr@ab-mart.com

订购专线:4006-123-828

销售电话:13916964679(微信同号)

技术支持:15618194176(微信同号)

华南经销商负责(广东,广西,福建,海南):
程经理:手机18616261485(微信同号)
华北经销商负责(北京,天津,河北):
徐经理:手机15618191473(微信同号)
南方经销商负责:

陆经理:手机13122837132(微信同号)
北方及西南经销商负责:

张经理:手机13122150513(微信同号)

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      微信客服

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