Plays an essential role in viral RNA synthesis and also a role in suppressing innate immune signaling . Acts as a polymerase cofactor in the RNA polymerase transcription and replication complexes . Serves as nucleoprotein/NP monomer chaperone prior to the formation of the large oligomeric RNA-bound complexes (By similarity). Regulates RNA synthesis by modulating NP-RNA interactions and interacting with DYNLL1 . VP35-NP interaction controls the switch between RNA-bound NP and free NP and thus the switch between genome replication and genome packaging into the nucleocapsid . Prevents establishment of cellular antiviral state, thereby suppressing host DC maturation . Acts by inhibiting host RIGI activation both by shielding dsRNA from detection and by preventing PRKRA binding to RIGI . Blocks virus-induced phosphorylation and activation of interferon regulatory factor 3/IRF3, a transcription factor critical for the induction of interferons alpha and beta . This blockage is produced through the interaction with and inhibition of host IKBKE and TBK1, producing a strong inhibition of the phosphorylation and activation of IRF3 . Also inhibits the antiviral effect mediated by the host interferon-induced, double-stranded RNA-activated protein kinase EIF2AK2/PKR . Increases PIAS1-mediated SUMOylation of IRF7, thereby repressing interferon transcription . Also acts as a suppressor of RNA silencing by interacting with host DICER1, TARBP2/TRBP and PRKRA/PACT (By similarity). As a dimer, binds and sequesters dsRNA contributing to the inhibition of interferon production (By similarity). .

q05127

Polymerase cofactor VP35 (Ebola VP35) (eVP35),VP35,VP35, Polymerase cofactor VP35, Ebola VP35, eVP35

Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus)

WB,ELISA

WB 1:500-1:2000
ELISA 1:5000-1:20000

38kD

Human

Monoclonal

F255

IgG

Protein A/G purified from cell culture supernatant.

>95% as determined by SDS-PAGE.

0.01M PBS, pH 7.4.

1 mg/ml

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.