[Fusion glycoprotein F0]: Inactive precursor that is cleaved at two sites by a furin-like protease to give rise to the mature F1 and F2 fusion glycoproteins. .; [Fusion glycoprotein F1]: Class I viral fusion protein . Under the current model, the protein has at least 3 conformational states: pre-fusion native state, pre-hairpin intermediate state, and post-fusion hairpin state . During viral and plasma cell membrane fusion, the coiled coil regions assume a trimer-of-hairpins structure, positioning the fusion peptide in close proximity to the C-terminal region of the ectodomain . The formation of this structure appears to drive apposition and subsequent fusion of viral and cellular membranes leading to delivery of the nucleocapsid into the cytoplasm . This fusion is pH independent and occurs at the plasma or endosomal membrane (Probable). The trimer of F1-F2 (F protein) also facilitates the attachment to host cell by binding to host heparan sulfate . F protein is involved in the entry into the host cell through the interaction with host IGF1R . This interaction activates PRKCZ/PKCzeta that recruits host NCL/nucleolin to the apical cell surface where it can bind fusion glycoprotein F1 . Later in infection, F protein expressed at the plasma membrane of infected cells can mediate fusion with adjacent cells to form syncytia, a cytopathic effect that could lead to tissue necrosis . F protein may trigger p53-dependent apoptosis . .; [Fusion glycoprotein F2]: Major determinant of the species specificity of RSV infection . The trimer of F1-F2 (F protein) also facilitates the attachment to host cell by binding to host heparan sulfate . F protein is involved in the entry into the host cell through the interaction with host IGF1R . This interaction activates PRKCZ/PKCzeta that recruits host NCL/nucleolin to the apical cell surface where it can bind fusion glycoprotein F1 . Later in infection, F protein expressed at the plasma membrane of infected cells can mediate fusion with adjacent cells to form syncytia, a cytopathic effect that could lead to tissue necrosis . F protein seems to trigger p53-dependent apoptosis . .

p03420

Fusion glycoprotein F0 [Cleaved into: Fusion glycoprotein F2 (F2); p27 (Intervening segment) (Pep27) (Peptide 27); Fusion glycoprotein F1 (F1)],F,Fusion glycoprotein F0, Fusion glycoprotein F2, F2, p27, Intervening segment, Pep27, Peptide 27, Fusion glycoprotein F1, F1, F

Human respiratory syncytial virus A (strain A2),Human respiratory syncytial virus B (strain B1)

ELISA,Blocking

ELISA 1:5000-1:20000
Blocking,The optimal dilutions should be determined by the end user.

64kD

Human

Monoclonal

RS265

IgG1, kappa

Protein A/G purified from cell culture supernatant.

>95% as determined by SDS-PAGE.

0.01M PBS, pH 7.4.

1 mg/ml

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.

For research use only. Not suitable for clinical or therapeutic use.