Type II interferon produced by immune cells such as T-cells and NK cells that plays crucial roles in antimicrobial, antiviral, and antitumor responses by activating effector immune cells and enhancing antigen presentation . Primarily signals through the JAK-STAT pathway after interaction with its receptor IFNGR1 to affect gene regulation . Upon IFNG binding, IFNGR1 intracellular domain opens out to allow association of downstream signaling components JAK2, JAK1 and STAT1, leading to STAT1 activation, nuclear translocation and transcription of IFNG-regulated genes. Many of the induced genes are transcription factors such as IRF1 that are able to further drive regulation of a next wave of transcription . Plays a role in class I antigen presentation pathway by inducing a replacement of catalytic proteasome subunits with immunoproteasome subunits . In turn, increases the quantity, quality, and repertoire of peptides for class I MHC loading . Increases the efficiency of peptide generation also by inducing the expression of activator PA28 that associates with the proteasome and alters its proteolytic cleavage preference . Up-regulates as well MHC II complexes on the cell surface by promoting expression of several key molecules such as cathepsins B/CTSB, H/CTSH, and L/CTSL . Participates in the regulation of hematopoietic stem cells during development and under homeostatic conditions by affecting their development, quiescence, and differentiation (By similarity). .

p01579

Interferon gamma (IFN-gamma) (Immune interferon),IFNG,Interferon gamma, IFN-gamma, Immune interferon, IFNG, IFN-γ

Human

ELISA,Neutralization

ELISA 1:5000-1:20000
Neutralization,The optimal dilutions should be determined by the end user.

20kD

Human

Monoclonal

Iv295

IgG1

Protein A/G purified from cell culture supernatant.

>95% as determined by SDS-PAGE.

0.01M PBS, pH 7.4.

1 mg/ml

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.

For research use only. Not suitable for clinical or therapeutic use.