Oligomerizes into helical capsid to encapsidate the viral genome, protecting it from nucleases and the cellular innate immune response. VP35 binds to and stabilizes monomeric NP, keeping it soluble. Upon virus replication, NP is recruited to bind cooperatively viral genomic RNA and VP35 is released. The encapsidated genomic RNA is termed the nucleocapsid and serves as template for transcription and replication. The nucleocapsid is helical with a pitch of 10.81 NP per turn and a diameter of about 22nm. Each NP binds to six nucleotides of viral genomic RNA, three being exposed to the solvant and three hidden into the nucleocapsid. Also recruits host PPP2R5C phosphatase to dephosphorylate VP30 and thereby promote viral transcription. Upon virion assembly and budding, NP binds to VP24 and possibly host STAU1. .

q8jpy1, p18272, b8xcm7, b8xcn6

Nucleoprotein (Nucleocapsid protein) (Protein N) (Reston NP) (rNP),NP,Nucleoprotein, Ebola NP, eNP, Nucleocapsid protein, Protein N, NP

Reston ebolavirus (strain Reston-89) (REBOV) (Reston Ebola virus), Zaire ebolavirus (strain Mayinga-76) (ZEBOV) (Zaire Ebola virus), Bundibugyo ebolavirus, Tai Forest ebolavirus

IF,ICC,ELISA,Neutralization,SPR

IF 1:100-1:500
ICC 1:100-1:400
ELISA 1:5000-1:20000
Neutralization,SPR,The optimal dilutions should be determined by the end user.

84kD

Human

Monoclonal

MJ266

IgG

Protein A/G purified from cell culture supernatant.

>95% as determined by SDS-PAGE.

0.01M PBS, pH 7.4.

1 mg/ml

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4℃ short term (1-2 weeks). Store at -20℃ 12 months.

For research use only. Not suitable for clinical or therapeutic use.