Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis . The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation . The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression . ; Deubiquitinates activated NOTCH1, promoting its nuclear import, thereby acting as a positive regulator of Notch signaling.

O00303

8665

Eukaryotic translation initiation factor 3 subunit F {ECO:0000255|HAMAP-Rule:MF_03005}, eIF3f {ECO:0000255|HAMAP-Rule:MF_03005}, Deubiquitinating enzyme eIF3f, Eukaryotic translation initiation factor 3 subunit 5 {ECO:0000255|HAMAP-Rule:MF_03005}, eIF-3-epsilon {ECO:0000255|HAMAP-Rule:MF_03005}, eIF3 p47 {ECO:0000255|HAMAP-Rule:MF_03005}, EIF3F {ECO:0000255|HAMAP-Rule:MF_03005}

Human,Mouse,Rat,Monkey

WB,IHC,FC

WB 1:500
IHC 1:50-1:200
FC 1:100

38 kD

Rabbit

Polyclonal

Rabbit IgG

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

PBS (pH7.4) with 0.05% (V/V) Proclin 300.

Store at -20°C. Stable for 12 months from date of receipt.Do not aliquot the antibody.Avoid freeze / thaw cycle.