Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis . Phosphorylates CABLES1, CTNNB1, CDK2AP2, ERCC6, NBN, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2 . Triggers duplication of centrosomes and DNA . Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus . Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in embryonic stem cells (ESCs) . Activity of CDK2 is maximal during S phase and G2; activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase . EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing . Cyclin E/CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC . Involved in G1-S phase DNA damage checkpoint that prevents cells with damaged DNA from initiating mitosis; regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis . In response to DNA damage, double-strand break repair by homologous recombination a reduction of CDK2-mediated BRCA2 phosphorylation . Involved in regulation of telomere repair by mediating phosphorylation of NBN . Phosphorylation of RB1 disturbs its interaction with E2F1 . NPM1 phosphorylation by cyclin E/CDK2 promotes its dissociates from unduplicated centrosomes, thus initiating centrosome duplication . Cyclin E/CDK2-mediated phosphorylation of NPAT at G1-S transition and until prophase stimulates the NPAT-mediated activation of histone gene transcription during S phase . Required for vitamin D-mediated growth inhibition by being itself inactivated . Involved in the nitric oxide- (NO) mediated signaling in a nitrosylation/activation-dependent manner . USP37 is activated by phosphorylation and thus triggers G1-S transition . CTNNB1 phosphorylation regulates insulin internalization . Phosphorylates FOXP3 and negatively regulates its transcriptional activity and protein stability (By similarity). Phosphorylates ERCC6 which is essential for its chromatin remodeling activity at DNA double-strand breaks . Acts as a regulator of the phosphatidylinositol 3-kinase/protein kinase B signal transduction by mediating phosphorylation of the C-terminus of protein kinase B (PKB/AKT1 and PKB/AKT2), promoting its activation .

P24941

1017

Cyclin-dependent kinase 2, Cell division protein kinase 2, p33 protein kinase, CDK2, CDKN2

Human,Mouse,Rat,Bovine,Hamster

WB

WB 1:1000

34 kD

Rabbit

Polyclonal

Rabbit IgG

This antibody is purified through a proteinA column.

PBS (pH7.4) with 0.05% (V/V) Proclin 300.

Store at -20°C. Stable for 12 months from date of receipt.Do not aliquot the antibody.Avoid freeze / thaw cycle.