Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.
注意:抗体应用为IHC的,抗体只适合于石蜡切片的组化染色或者荧光染色。
抗体应用为IF/ICC的,抗体适合于冰冻切片的组化染色或者荧光染色,以及细胞水平的化学染色和荧光染色。
ABMART实验方案下载
Acts as a negative regulator of entry into mitosis (G2 to M transition) by protecting the nucleus from cytoplasmically activated cyclin B1-complexed CDK1 before the onset of mitosis by mediating phosphorylation of CDK1 on ''Tyr-15'' . Specifically phosphorylates and inactivates cyclin B1-complexed CDK1 reaching a maximum during G2 phase and a minimum as cells enter M phase . Phosphorylation of cyclin B1-CDK1 occurs exclusively on ''Tyr-15'' and phosphorylation of monomeric CDK1 does not occur . Its activity increases during S and G2 phases and decreases at M phase when it is hyperphosphorylated . A correlated decrease in protein level occurs at M/G1 phase, probably due to its degradation .
P30291
7465
Wee1-like protein kinase, WEE1hu, Wee1A kinase, WEE1
Human
WB,IHC,Dot Blot
WB 1:1000IHC 1:50-1:200Dot Blot 1:2000-1:20000
72 kD
Rabbit
Polyclonal
Rabbit IgG
This antibody is purified through a proteinA column.
PBS (pH7.4) with 0.05% (V/V) Proclin 300.
Store at -20°C. Stable for 12 months from date of receipt.Do not aliquot the antibody.Avoid freeze / thaw cycle.
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