Serine kinase that plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses . Acts as a part of the canonical IKK complex in the conventional pathway of NF-kappa-B activation . Phosphorylates inhibitors of NF-kappa-B on 2 critical serine residues . These modifications allow polyubiquitination of the inhibitors and subsequent degradation by the proteasome . In turn, free NF-kappa-B is translocated into the nucleus and activates the transcription of hundreds of genes involved in immune response, growth control, or protection against apoptosis . In addition to the NF-kappa-B inhibitors, phosphorylates several other components of the signaling pathway including NEMO/IKBKG, NF-kappa-B subunits RELA and NFKB1, as well as IKK-related kinases TBK1 and IKBKE . IKK-related kinase phosphorylations may prevent the overproduction of inflammatory mediators since they exert a negative regulation on canonical IKKs . Phosphorylates FOXO3, mediating the TNF-dependent inactivation of this pro-apoptotic transcription factor . Also phosphorylates other substrates including NAA10, NCOA3, BCL10 and IRS1 . Phosphorylates RIPK1 at ''Ser-25'' which represses its kinase activity and consequently prevents TNF-mediated RIPK1-dependent cell death (By similarity). Phosphorylates the C-terminus of IRF5, stimulating IRF5 homodimerization and translocation into the nucleus . Following bacterial lipopolysaccharide (LPS)-induced TLR4 endocytosis, phosphorylates STAT1 at ''Thr-749'' which restricts interferon signaling and anti-inflammatory responses and promotes innate inflammatory responses . IKBKB-mediated phosphorylation of STAT1 at ''Thr-749'' promotes binding of STAT1 to the ARID5A promoter, resulting in transcriptional activation of ARID5A and subsequent ARID5A-mediated stabilization of IL6 . It also promotes binding of STAT1 to the IL12B promoter and activation of IL12B transcription .

O14920

3551

Inhibitor of nuclear factor kappa-B kinase subunit beta, I-kappa-B-kinase beta, IKK-B, IKK-beta, IkBKB, I-kappa-B kinase 2, IKK2, Nuclear factor NF-kappa-B inhibitor kinase beta, NFKBIKB, IKBKB, IKKB

Human

Dot Blot

Dot Blot 1:2000-1:20000

87 kD

Rabbit

Polyclonal

Rabbit IgG

This antibody is purified by peptide affinity purification.

PBS (pH7.4) with 0.09% (W/V) sodium azide.

Store at -20°C. Stable for 12 months from date of receipt.Do not aliquot the antibody.Avoid freeze / thaw cycle.