Chromatin reader component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA . Specifically recognizes and binds acylated histone H3, with a preference for histone H3 that is crotonylated . Crotonylation marks active promoters and enhancers and confers resistance to transcriptional repressors . Recognizes and binds histone H3 crotonylated at ''Lys-9'' (H3K9cr), and with slightly lower affinity histone H3 crotonylated at ''Lys-18'' (H3K18cr) . Also recognizes and binds histone H3 acetylated and butyrylated at ''Lys-9'' (H3K9ac and H3K9bu, respectively), but with lower affinity than crotonylated histone H3 . In the SEC complex, MLLT3 is required to recruit the complex to crotonylated histones . Recruitment of the SEC complex to crotonylated histones promotes recruitment of DOT1L on active chromatin to deposit histone H3 ''Lys-79'' methylation (H3K79me) . Plays a key role in hematopoietic stem cell (HSC) maintenance by preserving, rather than conferring, HSC stemness . Acts by binding to the transcription start site of active genes in HSCs and sustaining level of H3K79me2, probably by recruiting DOT1L .

P42568

4300

Protein AF-9, ALL1-fused gene from chromosome 9 protein, Myeloid/lymphoid or mixed-lineage leukemia translocated to chromosome 3 protein, YEATS domain-containing protein 3, MLLT3, AF9, YEATS3

Human,Mouse

WB,IHC

WB 1:1000
IHC 1:100-500

64 kD

Rabbit

Polyclonal

Rabbit IgG

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

PBS (pH7.4) with 0.09% (W/V) sodium azide.

Store at -20°C. Stable for 12 months from date of receipt.Do not aliquot the antibody.Avoid freeze / thaw cycle.