The TFIID basal transcription factor complex plays a major role in the initiation of RNA polymerase II (Pol II)-dependent transcription . TFIID recognizes and binds promoters with or without a TATA box via its subunit TBP, a TATA-box-binding protein, and promotes assembly of the pre-initiation complex (PIC) . The TFIID complex consists of TBP and TBP-associated factors (TAFs), including TAF1, TAF2, TAF3, TAF4, TAF5, TAF6, TAF7, TAF8, TAF9, TAF10, TAF11, TAF12 and TAF13 . The TFIID complex structure can be divided into 3 modules TFIID-A, TFIID-B, and TFIID-C . TBP forms the TFIID-A module together with TAF3 and TAF5 . TBP is a general transcription factor that functions at the core of the TFIID complex . During assembly of the core PIC on the promoter, as part of TFIID, TBP binds to and also bends promoter DNA, irrespective of whether the promoter contains a TATA box . Component of a BRF2-containing transcription factor complex that regulates transcription mediated by RNA polymerase III . Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC during RNA polymerase I-dependent transcription . The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter . SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA .

P20226

6908

TATA-box-binding protein, TATA sequence-binding protein, TATA-binding factor, TATA-box factor, Transcription initiation factor TFIID TBP subunit, TBP, GTF2D1, TF2D, TFIID

Human,Mouse,Bovine,Chicken,Xenopus,Monkey

WB,IHC,IF,ICC

WB 1:1000
IHC 1:100-500
IF 1:10-50
ICC 1:10-50

38 kD

Rabbit

Polyclonal

Rabbit IgG

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

PBS (pH7.4) with 0.09% (W/V) sodium azide.

Store at -20°C. Stable for 12 months from date of receipt.Do not aliquot the antibody.Avoid freeze / thaw cycle.