Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing. Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5''- and 3''-splice site binding components, U1 snRNP and U2AF. Can stimulate binding of U1 snRNP to a 5''-splice site-containing pre-mRNA. Binds to purine-rich RNA sequences, either the octamer, 5''-RGAAGAAC-3'' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC. Binds preferentially to the 5''-CGAGGCG-3'' motif in vitro. Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing. Isoform ASF-2 and isoform ASF-3 act as splicing repressors. May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway.

Q07955

6426

Serine/arginine-rich splicing factor 1, Alternative-splicing factor 1, ASF-1, Splicing factor, arginine/serine-rich 1, pre-mRNA-splicing factor SF2, P33 subunit, SRSF1, ASF, SF2, SF2P33, SFRS1

Human,Mouse,Bovine,Chicken,Pig,Zebrafish

WB,IHC,FC

WB 1:1000
IHC 1:100-500
FC 1:10-50

28 kD

Rabbit

Polyclonal

Rabbit IgG

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

PBS (pH7.4) with 0.09% (W/V) sodium azide.

Store at -20°C. Stable for 12 months from date of receipt.Do not aliquot the antibody.Avoid freeze / thaw cycle.