E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Specifically ubiquitinates ''Lys-63'' in target proteins . Involved in the pathway leading to the degradation of VEGFR-2/KDFR, independently of its ubiquitin-ligase activity. Monoubiquitinates IGF1R at multiple sites, thus leading to receptor internalization and degradation in lysosomes (By similarity). Ubiquitinates FGFR1, leading to receptor internalization and degradation in lysosomes . Promotes ubiquitination of RAPGEF2 . According to PubMed:18562292 the direct link between NEDD4 and PTEN regulation through polyubiquitination described in PubMed:17218260 is questionable. Involved in ubiquitination of ERBB4 intracellular domain E4ICD (By similarity). Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development (By similarity). Ubiquitinates TNK2 and regulates EGF-induced degradation of EGFR and TNF2 . Ubiquitinates BRAT1 and this ubiquitination is enhanced in the presence of NDFIP1 . Ubiquitinates DAZAP2, leading to its proteasomal degradation . Ubiquitinates POLR2A . Functions as a platform to recruit USP13 to form an NEDD4-USP13 deubiquitination complex that plays a critical role in cleaving the ''Lys-48''-linked ubiquitin chains of VPS34 and then stabilizing VPS34, thus promoting the formation of autophagosomes . ; (Microbial infection) Involved in the ubiquitination of Ebola virus protein VP40 which plays a role in viral budding.

P46934

4734

E3 ubiquitin-protein ligase NEDD4, 632-, Cell proliferation-inducing gene 53 protein, Neural precursor cell expressed developmentally down-regulated protein 4, NEDD-4, NEDD4, KIAA0093, NEDD4-1

Human,Mouse,Rat

WB,IHC,FC

WB 1:1000
IHC 1:100-500
FC 1:10-50

150 kD

Rabbit

Polyclonal

Rabbit IgG

This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.

PBS (pH7.4) with 0.09% (W/V) sodium azide.

Store at -20°C. Stable for 12 months from date of receipt.Do not aliquot the antibody.Avoid freeze / thaw cycle.