Orphan nuclear receptor that binds DNA as a monomer to the 5''-TCAAGGCCA-3'' sequence and controls expression of target genes: regulates key biological processes, such as early embryonic development, cholesterol and bile acid synthesis pathways, as well as liver and pancreas morphogenesis . Ligand-binding causes conformational change which causes recruitment of coactivators, promoting target gene activation . The specific ligand is unknown, but specific phospholipids, such as phosphatidylethanolamine, phosphatidylserine, dilauroyl phosphatidylcholine and diundecanoyl phosphatidylcholine can act as ligand in vitro . Acts as a pioneer transcription factor, which unwraps target DNA from histones and elicits local opening of closed chromatin . Plays a central role during preimplantation stages of embryonic development . Plays a minor role in zygotic genome activation (ZGA) by regulating a small set of two-cell stage genes . Plays a major role in morula development (2-16 cells embryos) by acting as a master regulator at the 8-cell stage, controlling expression of lineage-specifying transcription factors and genes involved in mitosis, telomere maintenance and DNA repair . Zygotic NR5A2 binds to both closed and open chromatin with other transcription factors, often at SINE B1/Alu repeats DNA elements, promoting chromatin accessibility at nearby regulatory regions . Also involved in the epiblast stage of development and embryonic stem cell pluripotency, by promoting expression of POU5F1/OCT4 . Regulates other processes later in development, such as formation of connective tissue in lower jaw and middle ear, neural stem cell differentiation, ovarian follicle development and Sertoli cell differentiation . Involved in exocrine pancreas development and acinar cell differentiation . Acts as an essential transcriptional regulator of lipid metabolism (By similarity). Key regulator of cholesterol 7-alpha-hydroxylase gene (CYP7A) expression in liver (By similarity). Activates the transcription of CYP2C38 . Also acts as a negative regulator of inflammation in different organs, such as intestine, liver and pancreas . Protects against intestinal inflammation via its ability to regulate glucocorticoid production . Plays an anti-inflammatory role during the hepatic acute phase response by acting as a corepressor: inhibits the hepatic acute phase response by preventing dissociation of the N-Cor corepressor complex (By similarity). Acts as a regulator of immunity by promoting lymphocyte T-cell development, proliferation and effector functions . Also involved in resolution of endoplasmic reticulum stress in the liver .

P45448

26424

Nuclear receptor subfamily 5 group A member 2, Liver receptor homolog 1, LRH-1, Nr5a2, Lrh1

Human,Mouse,Rat

WB

WB 1:1000

65 kD

Rabbit

polyclonal

Rabbit IgG

This antibody is purified by peptide affinity purification.

PBS (pH7.4) with 0.09% (W/V) sodium azide.

Store at -20°C. Stable for 12 months from date of receipt.Do not aliquot the antibody.Avoid freeze / thaw cycle.