E3 ubiquitin-protein ligase that mediates monoubiquitination of ''Lys-119'' of histone H2A (H2AK119Ub), thereby playing a central role in histone code and gene regulation . H2AK119Ub gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals . May be involved in the initiation of both imprinted and random X inactivation . Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development . PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility . E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4 . Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity . Plays a role in the transcriptional repression of genes that are required for pluripotency in embryonic stem cells, thereby contributing to differentiation of the ectodermal and endodermal germ layers . Association with the chromosomal DNA is cell-cycle dependent. In resting B- and T-lymphocytes, interaction with AURKB leads to block its activity, thereby maintaining transcription in resting lymphocytes . Also acts as a negative regulator of autophagy by mediating ubiquitination of AMBRA1, leading to its subsequent degradation .

Q9CQJ4

19821

E3 ubiquitin-protein ligase RING2, 632-, RING finger protein 1B, RING1b, RING finger protein 2, Rnf2, DinG, Ring1b

Human,Mouse,Rat

WB

WB 1:2000

38 kD

Rabbit

polyclonal

Rabbit IgG

This antibody is purified by peptide affinity purification.

PBS (pH7.4) with 0.09% (W/V) sodium azide.

Store at -20°C. Stable for 12 months from date of receipt.Do not aliquot the antibody.Avoid freeze / thaw cycle.