Anti-apoptotic protein known as inhibitor of apoptosis (IAP) which can regulate cell death by controlling caspases and by acting as an E3 ubiquitin-protein ligase . Unlike most IAPs, does not contain a RING domain and it is not a RING-type E3 ligase . Instead acts as a dual E2/E3 enzyme that combines ubiquitin conjugating (E2) and ubiquitin ligase (E3) activities in a single polypeptide . Ubiquitination is mediated by a non-canonical E1 ubiquitin activating enzyme UBA6 . Ubiquitinates CASP3, CASP7 and CASP9 and inhibits their caspase activity; also ubiquitinates their procaspases but to a weaker extent . Ubiquitinates pro-apoptotic factors DIABLO/SMAC and HTRA2 . DIABLO/SMAC antagonizes the caspase inhibition activity of BIRC6 by competing for the same binding sites as the caspases . Ubiquitinates the autophagy protein MAP1LC3B; this activity is also inhibited by DIABLO/SMAC . Important regulator for the final stages of cytokinesis . Crucial for normal vesicle targeting to the site of abscission, but also for the integrity of the midbody and the midbody ring, and its striking ubiquitin modification .

Q9NR09

57448

Baculoviral IAP repeat-containing protein 6, 6.3.2.-, BIR repeat-containing ubiquitin-conjugating enzyme, BRUCE, Ubiquitin-conjugating BIR domain enzyme apollon, APOLLON, BIRC6, KIAA1289

Human,Mouse

WB,FC

WB 1:1000
FC 1:25

531 kD

Rabbit

polyclonal

Rabbit IgG

This antibody is purified by peptide affinity purification.

PBS (pH7.4) with 0.09% (W/V) sodium azide.

Store at -20°C. Stable for 12 months from date of receipt.Do not aliquot the antibody.Avoid freeze / thaw cycle.