F4/80 antigen is a 160 kDa cell surface glycoprotein that is a member of the EGF TM7 family of proteins and shares 68% overall amino acid identity with human EGF module containing mucin like hormone receptor 1 (EMR1). Expression of F4/80 is heterogeneous and is reported to vary during macrophage maturation and activation. The F4/80 antigen is expressed on a wide range of mature tissue macrophages including Kupffer cells, Langerhans, microglia, macrophages located in the gut lamina propria, peritoneal cavity, lung, thymus, bone marrow stroma and macrophages in the red pulp of the spleen. F4/80 expression has also been reported on a subpopulation of dendritic cells but is absent from macrophages located in T cell areas of the spleen and lymph node. The ligands and biological functions of the F4/80 antigen have not yet been determined but recent studies suggest a role for F4/80 in the generation of efferent CD8+ve regulatory T cells.

Adhesion G protein-coupled receptor E1; Cell surface glycoprotein F4/80; EGF-like module containing, mucin-like, hormone receptor-like sequence 1; EGF-like module receptor 1; EGF-like module-containing mucin-like hormone receptor-like 1; EMR1 hormone receptor; lymphocyte antigen 71, EMR 1; EMR1 hormone receptor; Gpf480; Ly71; Lymphocyte antigen 71; TM7LN3;Adgre1; DD7A5-7; EGF-TM7; Emr1; F4/80; Gpf480; Ly71; TM7LN3.

Q14246

Goat

Human,Mouse,Rat

WB,IF,ICC

WB 1:500-1000
IF 1:100-300
ICC 1:100-300

120kD

Recombinant Human F4/80

Ammonium sulfate precipitation&Antigen affinity chromatography

~1mg/ml

Liquid

PBS, 50% glycerol, 0.05% Proclin 300, 0.08%BSA

Store at -20 °C. Stable for one year