function:May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts and smooth muscle but not endothelial cells. It is able to bind EGF receptors with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.,PTM:O-linked glycan attachment sites were determined by Edman degradation, O-glycanase digest suggests mucin-type glycosylation (done in HB-EGF purified from histiocytic lymphoma cell line U-937).,PTM:Several N-termini have been identified by direct sequencing. The forms with N-termini 63, 73 and 74 have been tested and found to be biologically active.,similarity:Contains 1 EGF-like domain.,subcellular location:Mature HB-EGF is released into the extracellular space and probably binds to a receptor.,

Proheparin-binding EGF-like growth factor [Cleaved into: Heparin-binding EGF-like growth factor (HB-EGF) (HBEGF) (Diphtheria toxin receptor) (DT-R)],DTR;DTS;HEGFL,HBEGF

Q99075,Q06186,Q06175

Human,Mouse,Rat

WB,IHC,IF,ICC,ELISA

WB 1:1000-1:5000
IHC 1:200-1:1000
IF 1:200-1:1000
ICC 1:200-1:1000
ELISA 1:5000-1:20000

21kDa

[Heparin-binding EGF-like growth factor]: Secreted, extracellular space. Mature HB-EGF is released into the extracellular space and probably binds to a receptor.; [Proheparin-binding EGF-like growth factor]: Cell membrane; Single-pass type I membrane protein.

Rabbit

Monoclonal

IgG,Kappa

PBS, 50% glycerol, 0.05% Proclin 300, 0.05%BSA

-20°C/1 year(Do not lower than -25°C)