A cytochrome P450 monooxygenase involved in the metabolism of sterols, steroid hormones, retinoids and fatty acids . Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (NADPH--hemoprotein reductase). Catalyzes the hydroxylation of carbon-hydrogen bonds . Exhibits high catalytic activity for the formation of hydroxyestrogens from estrone (E1) and 17beta-estradiol (E2), namely 2-hydroxy E1 and E2, as well as D-ring hydroxylated E1 and E2 at the C-16 position . Plays a role in the metabolism of androgens, particularly in oxidative deactivation of testosterone . Metabolizes testosterone to less biologically active 2beta- and 6beta-hydroxytestosterones . Contributes to the formation of hydroxycholesterols (oxysterols), particularly A-ring hydroxylated cholesterol at the C-4beta position, and side chain hydroxylated cholesterol at the C-25 position, likely contributing to cholesterol degradation and bile acid biosynthesis . Catalyzes bisallylic hydroxylation of polyunsaturated fatty acids (PUFA) . Catalyzes the epoxidation of double bonds of PUFA with a preference for the last double bond . Metabolizes endocannabinoid arachidonoylethanolamide (anandamide) to 8,9-, 11,12-, and 14,15-epoxyeicosatrienoic acid ethanolamides (EpETrE-EAs), potentially modulating endocannabinoid system signaling . Plays a role in the metabolism of retinoids. Displays high catalytic activity for oxidation of all-trans-retinol to all-trans-retinal, a rate-limiting step for the biosynthesis of all-trans-retinoic acid (atRA) . Further metabolizes atRA toward 4-hydroxyretinoate and may play a role in hepatic atRA clearance . Responsible for oxidative metabolism of xenobiotics. Acts as a 2-exo-monooxygenase for plant lipid 1,8-cineole (eucalyptol) . Metabolizes the majority of the administered drugs. Catalyzes sulfoxidation of the anthelmintics albendazole and fenbendazole . Hydroxylates antimalarial drug quinine . Acts as a 1,4-cineole 2-exo-monooxygenase . Also involved in vitamin D catabolism and calcium homeostasis. Catalyzes the inactivation of the active hormone calcitriol (1-alpha,25-dihydroxyvitamin D(3)) . .

Cytochrome P450 3A4 (EC 1.14.14.1) (1,4-cineole 2-exo-monooxygenase) (1,8-cineole 2-exo-monooxygenase) (EC 1.14.14.56) (Albendazole monooxygenase (sulfoxide-forming)) (EC 1.14.14.73) (Albendazole sulfoxidase) (CYPIIIA3) (CYPIIIA4) (Cholesterol 25-hydroxylase) (Cytochrome P450 3A3) (Cytochrome P450 HLp) (Cytochrome P450 NF-25) (Cytochrome P450-PCN1) (Nifedipine oxidase) (Quinine 3-monooxygenase) (EC 1.14.14.55) CYP3A4 CYP3A3

Human

WB

WB 1:500-1:1000

57 KD

CYP3A4 CYP3A3

p08684/p20815

https://www.uniprot.org/uniprotkb/p08684

Rabbit

Polyclonal

Rabbit IgG

Transmembrane

Cholesterol metabolism #Lipid metabolism #Steroid metabolism

PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol

Store at -20 °C. Stable for 12 months from date of receipt.