Lysine acetylation is an important reversible modification controlling protein activity. n various species, acetylationof lysine residues is an important form of protein posttranslational modification(PTM) that affects thousands ofproteins such as Histones, transcript factors, lFN receptors, as well as P53, tubulin, Hsp90 that ranging from nuclearregulators to cytosolic proteins, mitochondrial enzymes, and plasma membrane associated receptors ll) Theregulation of protein acetylation is involved in control of cell cycle, metabolism, cell signaling, nuclear transport andeven other PTM process (1.2.3).

[1]:Xiang-iao Y.et al.(2008)Molecular Cell 31,449-461.

[2]:Choudhary C.et al.(2014)Nature Reviews Molecular Cell Biology15, 536-550.

[3]:Choudhary,C.et al.(2009)Science 325.834-40.

Target           Acetylated-Lysine

Host              All species

Reactivity      All species

lsotype         Mouse lgG

IMPORTANT：Use an anti-MOUSE secondary antibody to detect the Acetylated-Lysine Mab

WB              1:200-1:1000

IP                 5-10ul per reaction(total 500-1000ug lysate)

IP- LC/MS    20-50ul per reaction(total 2000-5000ug lysate）

Fig1 Western blot analysis of chemical acetylated 293T totallysate (10ug, lane1), un-acetylated 293T total lysate (10ug. lane2),chemical acetylated BSA (5ng, lane 3) and un-acetylatedBSA (5ng, lane 4), using Abmart Acetylated-Lysine Mab at a1000 dilution.

Fig.2 Relative distribution of acetylated proteins withdiverse biological process identifed from an immunoprecipitation LC-MS/MS experiment of mouse livertissue using Acetylated-Lysine Mab

Please add Milil-Q water or Abmart solution buffer to the tube before use, at the concentration ofl mg/ml.