Histone H4 Antibody_经典一抗 -

产品类型

蛋白质研究服务
目录抗体
经典一抗
二抗
标签/内参抗体
Beads抗体
病理诊断与药物研发
直标流式抗体
模式小物种抗体
辅助产品
小分子抑制剂
核酸研究试剂
细胞因子
重组蛋白
酶标试剂盒

PA3472

货号规格价格(￥)

PA3472S 50μl

PA3472M 100μl

以上产品均有现货供应

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Note: Application as IHC, only suitable for histochemical staining or fluorescence staining of paraffin-embedded sections. Application as ICC/IF, suitable for histochemical or fluorescent staining of frozen sections, as well as chemical and fluorescent staining at the cellular level.

注意：抗体应用为IHC的，抗体只适合于石蜡切片的组化染色或者荧光染色。

抗体应用为IF/ICC的，抗体适合于冰冻切片的组化染色或者荧光染色，以及细胞水平的化学染色和荧光染色。

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经典一抗-Histone H4 Antibody

Datasheet

Background

Rabbit polyclonal antibody to Histone H4

Alternative Name

Histone H4 H4C1 H4/A H4FA HIST1H4A; H4C2 H4/I H4FI HIST1H4B; H4C3 H4/G H4FG HIST1H4C; H4C4 H4/B H4FB HIST1H4D; H4C5 H4/J H4FJ HIST1H4E; H4C6 H4/C H4FC HIST1H4F; H4C8 H4/H H4FH HIST1H4H; H4C9 H4/M H4FM HIST1H4I; H4C11 H4/E H4FE HIST1H4J; H4C12 H4/D H4FD HIST1H4K; H4C13 H4/K H4FK HIST1H4L; H4C14 H4/N H4F2 H4FN HIST2H4 HIST2H4A; H4C15 H4/O H4FO HIST2H4B; H4C16 H4-16 HIST4H4

Reactivity

Human,Mouse,Rat,Bovine,Chicken,Pig

Predicted Reactivity

Application

WB,IHC,IF,ICC

Application Image

Recommended Dilution

WB 1:500-1:1000
IHC 1:50-1:200
IF 1:100-1:400
ICC 1:100-1:400

Mol Weight

12kD

Gene Symbol

HIST1H4A; HIST1H4B; HIST1H4C; HIST1H4D; HIST1H4E; HIST1H4F; HIST1H4H; HIST1H4I; HIST1H4J; HIST1H4K; HIST1H4L; HIST2H4A; HIST2H4B; HIST4H4

Uniprot

p62805

Uniprot link

https://www.uniprot.org/uniprotkb/p62805

Immunogen

Immunogen Range

Source

Rabbit

Clonality

Polyclonal

Isotype

Rabbit IgG

Specificity

Recognizes endogenous levels of Histone H4 protein.

Subcellular location

Nucleus localization

Research Area

chromatin organization

Positive control

Conjugation

Modification

Purification

Formulation

Concentration

Buffer

PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

产品介绍

Clone

Target

波长

Storage

Store at -20°C long term.

注意事项

Companion Products

Important

Comments

RRID

客户评价

WB
IHC
IF/ICC

Western blot analysis of Histone H4 expression in Hela (A), H1688 (B), mouse lung (C), mouse liver (D), rat liver (E) whole cell lysates.

Immunohistochemical analysis of Histone H4 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of Histone H4 staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.