Serine/threonine-protein kinase involved in the control of the cell cycle; essential for meiosis, but dispensable for mitosis . Phosphorylates CABLES1, CTNNB1, CDK2AP2, ERCC6, NBN, USP37, p53/TP53, NPM1, CDK7, RB1, BRCA2, MYC, NPAT, EZH2 . Triggers duplication of centrosomes and DNA . Acts at the G1-S transition to promote the E2F transcriptional program and the initiation of DNA synthesis, and modulates G2 progression; controls the timing of entry into mitosis/meiosis by controlling the subsequent activation of cyclin B/CDK1 by phosphorylation, and coordinates the activation of cyclin B/CDK1 at the centrosome and in the nucleus . Crucial role in orchestrating a fine balance between cellular proliferation, cell death, and DNA repair in embryonic stem cells (ESCs) . Activity of CDK2 is maximal during S phase and G2; activated by interaction with cyclin E during the early stages of DNA synthesis to permit G1-S transition, and subsequently activated by cyclin A2 (cyclin A1 in germ cells) during the late stages of DNA replication to drive the transition from S phase to mitosis, the G2 phase . EZH2 phosphorylation promotes H3K27me3 maintenance and epigenetic gene silencing . Cyclin E/CDK2 prevents oxidative stress-mediated Ras-induced senescence by phosphorylating MYC . Involved in G1-S phase DNA damage checkpoint that prevents cells with damaged DNA from initiating mitosis; regulates homologous recombination-dependent repair by phosphorylating BRCA2, this phosphorylation is low in S phase when recombination is active, but increases as cells progress towards mitosis . In response to DNA damage, double-strand break repair by homologous recombination a reduction of CDK2-mediated BRCA2 phosphorylation . Involoved in regulation of telomere repar by mediating phosphorylation of NBN . Phosphorylation of RB1 disturbs its interaction with E2F1 . NPM1 phosphorylation by cyclin E/CDK2 promotes its dissociates from unduplicated centrosomes, thus initiating centrosome duplication . Cyclin E/CDK2-mediated phosphorylation of NPAT at G1-S transition and until prophase stimulates the NPAT-mediated activation of histone gene transcription during S phase . Required for vitamin D-mediated growth inhibition by being itself inactivated . Involved in the nitric oxide- (NO) mediated signaling in a nitrosylation/activation-dependent manner . USP37 is activated by phosphorylation and thus triggers G1-S transition . CTNNB1 phosphorylation regulates insulin internalization . Phosphorylates FOXP3 and negatively regulates its transcriptional activity and protein stability (By similarity). Phosphorylates ERCC6 which is essential for its chromatin remodeling activity at DNA double-strand breaks . .

Cyclin-dependent kinase 2 (EC 2.7.11.22) (Cell division protein kinase 2) (p33 protein kinase) CDK2 CDKN2

Mouse;Rat;Pig;Human

WB,IHC,IF,ICC

WB 1:300-1:1000
IHC 1:50-1:200
IF 1:100-1:400
ICC 1:100-1:400

33.930kD;

CDK2 CDKN2

p24941

https://www.uniprot.org/uniprotkb/p24941

RKRB88953-Recombinant Cyclin Dependent Kinase 2 (CDK2) Ser94~Thr198 (Accession # P24941)

Rabbit

Polyclonal

Rabbit IgG

Enzyme Kinase;Metabolic pathway;Developmental science;

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Liquid

500μg/mL

0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Store at +4℃ after thawing. For long-term storage, please store it at -20℃. Avoid repeated freeze / thaw cycles.