Molecular adapter required for selective macroautophagy (aggrephagy) by acting as a a bridge between polyubiquitinated proteins and autophagosomes . Promotes the recruitment of ubiquitinated cargo proteins to autophagosomes via multiple domains that bridge proteins and organelles in different steps . SQSTM1 first mediates the assembly and removal of ubiquitinated proteins by undergoing liquid-liquid phase separation upon binding to ubiquitinated proteins via its UBA domain, leading to the formation of insoluble cytoplasmic inclusions, known as p62 bodies . SQSTM1 then interacts with ATG8 family proteins on autophagosomes via its LIR motif, leading to p62 body recruitment to autophagosomes, followed by autophagic clearance of ubiquitinated proteins . SQSTM1 is itself degraded along with its ubiquitinated cargos . Also required to recruit ubiquitinated proteins to PML bodies in the nucleus . Also involved in autophagy of peroxisomes (pexophagy) in response to reactive oxygen species (ROS) by acting as a bridge between ubiquitinated PEX5 receptor and autophagosomes . Acts as an activator of the NFE2L2/NRF2 pathway via interaction with KEAP1: interaction inactivates the BCR(KEAP1) complex by sequestering the complex in inclusion bodies, promoting nuclear accumulation of NFE2L2/NRF2 and subsequent expression of cytoprotective genes . Promotes relocalization of 'Lys-63'-linked ubiquitinated STING1 to autophagosomes . Involved in endosome organization by retaining vesicles in the perinuclear cloud: following ubiquitination by RNF26, attracts specific vesicle-associated adapters, forming a molecular bridge that restrains cognate vesicles in the perinuclear region and organizes the endosomal pathway for efficient cargo transport . Sequesters tensin TNS2 into cytoplasmic puncta, promoting TNS2 ubiquitination and proteasomal degradation . May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1 . May play a role in titin/TTN downstream signaling in muscle cells . Adapter that mediates the interaction between TRAF6 and CYLD (By similarity). .

Sequestosome-1 (EBI3-associated protein of 60 kDa) (EBIAP) (p60) (Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa) (Ubiquitin-binding protein p62) SQSTM1 ORCA OSIL

Rat;Human

WB,IHC,IF,ICC

WB 1:70-1:700
IHC 1:50-1:200
IF 1:100-1:400
ICC 1:100-1:400

47.687kD;

SQSTM1 ORCA OSIL

q13501

https://www.uniprot.org/uniprotkb/q13501

RKRD19842-Recombinant Sequestosome 1 (SQSTM1) Val136~Gln400

Rabbit

Polyclonal

Rabbit IgG

Signal transduction;

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Liquid

500μg/mL

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Store at +4℃ after thawing. For long-term storage, please store it at -20℃. Avoid repeated freeze / thaw cycles.