E3 ubiquitin-protein ligase involved in different protein quality control pathways in the cytoplasm . Component of the N-end rule pathway: ubiquitinates proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their degradation . Recognizes both type-1 and type-2 N-degrons, containing positively charged amino acids (Arg, Lys and His) and bulky and hydrophobic amino acids, respectively . Does not ubiquitinate proteins that are acetylated at the N-terminus . Together with UBR5, part of a cytoplasm protein quality control pathway that prevents protein aggregation by catalyzing assembly of heterotypic 'Lys-11'-/'Lys-48'-linked branched ubiquitin chains on aggregated proteins, leading to substrate recognition by the segregase p97/VCP and degradation by the proteasome: UBR4 probably synthesizes mixed chains containing multiple linkages, while UBR5 is likely branching multiple 'Lys-48'-linked chains of substrates initially modified . Together with KCMF1, part of a protein quality control pathway that catalyzes ubiquitination and degradation of proteins that have been oxidized in response to reactive oxygen species (ROS): recognizes proteins with an Arg-CysO3(H) degron at the N-terminus, and mediates assembly of heterotypic 'Lys-63'-/'Lys-27'-linked branched ubiquitin chains on oxidized proteins, leading to their degradation by autophagy . Catalytic component of the SIFI complex, a multiprotein complex required to inhibit the mitochondrial stress response after a specific stress event has been resolved: ubiquitinates and degrades (1) components of the HRI-mediated signaling of the integrated stress response, such as DELE1 and EIF2AK1/HRI, as well as (2) unimported mitochondrial precursors . Within the SIFI complex, UBR4 initiates ubiquitin chain that are further elongated or branched by KCMF1 . Mediates ubiquitination of ACLY, leading to its subsequent degradation . Together with clathrin, forms meshwork structures involved in membrane morphogenesis and cytoskeletal organization . .

E3 ubiquitin-protein ligase UBR4 (EC 2.3.2.27) (600 kDa retinoblastoma protein-associated factor) (p600) (N-recognin-4) (Retinoblastoma-associated factor of 600 kDa) (RBAF600) UBR4 KIAA0462 KIAA1307 RBAF600

Mouse;Rat;Pig;Human

WB,IHC

WB 1:300-1:1000
IHC 1:50-1:200

573.841kD;

UBR4 KIAA0462 KIAA1307 RBAF600

q5t4s7

https://www.uniprot.org/uniprotkb/q5t4s7

RKRL07479-Recombinant Ubiquitin Protein Ligase E3 Component N-Recognin 4 (UBR4) Val124~Arg396

Rabbit

Polyclonal

Rabbit IgG

Enzyme Kinase;

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Liquid

500μg/mL

PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.

Store at +4℃ after thawing. For long-term storage, please store it at -20℃. Avoid repeated freeze / thaw cycles.